1O1415 Force generation by single and multiple molecules of single-headed kinesin
نویسندگان
چکیده
منابع مشابه
Energetics of the single-headed kinesin KIF1A.
KIF1A is a single-headed molecular motor that moves processively and unidirectionally along a microtubule by using the chemical energy released by hydrolyzing adenosine triphosphate (ATP) into adenosine diphosphate (ADP) and inorganic phosphate (P(i)). Although the movement of KIF1A seems to have successfully been explained by a simple Brownian motor model of the flashing ratchet type, this mod...
متن کاملForce and velocity measured for single kinesin molecules.
We measured the force-velocity curves of single kinesin molecules attached to silica beads moving in an in vitro motility assay. Optical trapping interferometry was used to track movement with subnanometer precision and to apply calibrated, pN-sized forces to the beads. Velocity decreased linearly with increasing force, and kinesin molecules moved against applied loads of up to 5-6 pN. Comparis...
متن کاملAn improved optical tweezers assay for measuring the force generation of single kinesin molecules.
Numerous microtubule-associated molecular motors, including several kinesins and cytoplasmic dynein, produce opposing forces that regulate spindle and chromosome positioning during mitosis. The motility and force generation of these motors are therefore critical to normal cell division, and dysfunction of these processes may contribute to human disease. Optical tweezers provide a powerful metho...
متن کاملIntracellular transport by single-headed kinesin KIF1A: effects of single-motor mechanochemistry and steric interactions.
In eukaryotic cells, many motor proteins can move simultaneously on a single microtubule track. This leads to interesting collective phenomena such as jamming. Recently we reported [Phys. Rev. Lett. 95, 118101 (2005)] a lattice-gas model which describes traffic of unconventional (single-headed) kinesins KIF1A. Here we generalize this model, introducing an interaction parameter c, to account for...
متن کاملResolving Single Kinesin Motors in Motion by Atomic Force Microscopy
Carolina Carrasco, Iwan A. T. Schaap, Pedro J. de Pablo, Christoph f. Schmidt 1 Departamento de Física de la Materia Condensada C-III, Universidad Autónoma de Madrid, 28049 Madrid, Spain 2 National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA, United Kingdom 3 Physikalisches Institut, Fakultät für Physik, Georg-August-Universität, Friedrich-Hund-Platz 1, 37077 Göttin...
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ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 2002
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.42.s90_3